Comprehensive Guide to Hydrolysis and Analysis of Amino Acids

This guide is offered as an overview of the sample preparation considerations behind reliable, amino acid analysis of peptides and proteins contained in several common sources. The material can be used as a starting point for those who are performing amino acid analysis, as well as a resource for experienced analysts who are trying to resolve a related issue. Included is a list of select references that contain additional information on some of the discussed topics. The detailed procedures were obtained primarily from specific publications, AOAC official methods, and Waters Corporation’s 30 years of amino acid analysis experience. The overriding goal was to ensure likely success by presenting methods that carry levels of robustness when used as detailed in the document. Most of the included procedures have been used by analysts throughout the years. The hope is that these selections address many application needs.

In this Primer

Comprehensive Guide to Hydrolysis and Analysis of Amino Acids

Introduction to Hydrolysis

Hydrolysis of Purified Proteins and Peptides

Hydrolysis of Food and Feed Samples

Operation of the Eldex Hydrolysis/Derivatization Workstation for Liquid- and Vapor-Phase Hydrolysis

Operation of the CEM Discover SP Microwave Reaction System for Amino Acid Hydrolysis

Derivatization of Amino Acids Using Waters AccQ•Tag Chemistry

Quantitation of Amino Acids

Summary