Standard test tube (13.0 to 18.0 mm) rack
Eppendorf tube rack
Scintillation vial (16.5 mm) rack
Scintillation vial (27.5 mm) rack
Microtiter plate (4 x 96/384) rack
Preparative funnel (4 x 32 positions) rack
Multipurpose rack
Collecting purified material from your liquid chromatography (LC) system, especially in a user-friendly and cost-effective way, can be a challenge.
Waters Fraction Collector III is an easy-to-use, standalone, or software-controlled fraction collector designed for use with high performance liquid chromatography (HPLC) and LC-MS applications. Compatible with flow rates as high as 300 mL per minute, the Fraction Collector III handles a wide range of adapter racks and related collection vessels. Its flexible design accommodates a variety of fraction sizes, whether you are collecting to microtiter plates or multiple containers.
Standard test tube (13.0 to 18.0 mm) rack
Eppendorf tube rack
Scintillation vial (16.5 mm) rack
Scintillation vial (27.5 mm) rack
Microtiter plate (4 x 96/384) rack
Preparative funnel (4 x 32 positions) rack
Multipurpose rack
Methods |
Time, drop count, drop volume, signal |
Nozzle shift time |
0.1 s (tube center to center) |
Simple mode |
Time |
99 min 59 s/tube max. |
Drop |
9999 count/tube max. |
|
Volume |
999.9 mL/tube max. |
|
Signal |
9999 count/tube max. |
|
Peak mode |
End time |
999 min max. |
Request time |
99 min 59 s/tube max. |
|
Monitor time |
99 min 59 s/tube max. |
|
Fraction peak |
Up to 10 out of 99 peaks |
|
Peak discrimination |
Absolute value 100% max. Slope 100 mV/min or 10 V/min max. |
|
Window mode |
Non-window monitor |
99 min 59 s max. |
No. of windows |
10 max. |
|
Start/end time |
999 min 59 s max. |
|
Request/monitor time |
99 min 59 s/tube max. |
|
Manual mode |
Learning response |
Chromatogram signal display High 0.5 s; low 3.0 s |
Sampling mode |
Playback |
Replay results of learning |
Interval |
99 min 59 s max. |
|
Collection time |
99 min 59 s max. |
|
Collection volume |
999.9 mL max. |
|
Number of repeats |
99 max. |
Event markers |
2 contact point output circuits |
|
Signal input |
10 mV or 1 V full scale |
|
External start input |
On start |
|
External end input |
On end |
|
External count input |
On count |
|
Serial interface |
RS-232C (9-pin connector) |
|
Power requirements |
100.00 V AC 115.00 V AC 240.00 V AC 0.23 A max. |
|
Local user interface |
Operation display |
40 characters by 8 rows (LCD w/backlight) |
Parameter memory |
Battery back-up |
|
Remote control |
PC control with MassLynx and Empower 2 Software |
External dimensions |
Width: 28.60 cm (11.27 in.) Depth: 35.90 cm (14.14 in.) Height: 33.50 cm (13.20 in.) |
Weight |
Approximately 7.00 kg (15.40 lbs) |
Temperature range |
2 to 40 °C (35 to 104 °F) |
Title |
Part Number |
Waters Fraction Collector III (Includes one standard rack that holds up to 120 tubes; 12 to 18 mm O.D. x 90 to 180 mm height) |
186001878 |
Diverter valve options |
|
Divert valve assembly, standard |
725000142 |
Divert valve assembly, low volume (Recommended for microtiter plate rack) |
725000143 |
Fittings kit (Drop tubes and connecting tubing to accommodate different flow rates) |
205000131 |
Rack options |
|
Microtiter plate rack (Accepts four microtiter plates, standard, and deep-well) |
725000110 |
Dropper extension nozzle (Recommended for microtiter plate rack) |
725000151 |
Eppendorf tube rack (accepts up to 120 eppendorf tubes) |
725000145 |
Title |
Part Number |
Scintillation vial rack, 16.5 mm (accepts up to 120 scintillation vials; 16.5 mm O.D. x 54.0 mm height) |
725000146 |
Scintillation vial rack, 27.5 mm (accepts up to 56 scintillation vials; 27.5 mm O.D. x 57.0 mm height) |
725000147 |
Multipurpose rack |
725000113 |
Preparative funnel adapter rack (used with preparative funnel adapters – up to four) |
725000107 |
Preparative funnel adapter, 2 per package (collects up to 32 fractions; requires tubing to connect adapter to collection vessels) |
725000106 |
Tubing for preparative funnel adapter, Tygon 50 feet |
WAT037047 |
Tubing for preparative funnel adapter, Teflon 50 feet |
WAT037090 |
Table-top shelf assembly, for use with prep funnel rack |
289000440 |
Recommended Use: For flexible collection of a variety of fraction sizes, designed for use with HPLC and LC-MS applications.
Fraction Collector III offers several simple to advanced modes of operation for fraction collection, including time, threshold, volume, manual, and sampling. These modes of operation can be combined to allow for advanced fraction collection, such as pooling of repeated runs into common vessels.
Peak detection and collection using an analog detector input from a liquid chromatography (LC) system permits fully automated collection of the peaks of interest. The diverter valve in the system enables you to collect peaks without extraneous solvent and/or unwanted peaks (which are diverted to waste), maximizing fraction collection efficiency and overall preparative LC system productivity.
For maximum control and usability, you can control the Fraction Collector III from a local keypad or through serial port communication by Empower 3 or MassLynx Software.
Control of the Fraction Collector III is accessible through a new instrument control software interface that integrates seamlessly with Empower Software control components. The user can use the interface to configure collection vessels and program collection routines. Real-time feedback from the Fraction Collector III indicates the state of the collection process and the location of the collected fractions.
Multiple collector rack configurations (including custom configurations) accommodate a variety of collection vessels. If the user needs to override collection control, the interface enables software-assisted manual collection through its override function, directly from the instrument control panel.