ACQUITY Arc Multi-Dimensional Liquid Chromatography (MDLC) System

Solvent capacity

Blend up to four solvents in any combination (standard); total capacity of nine solvents with integrated solvent select valve (optional)

Number of fluidic paths

Three (Path 1, Path 2, and waste), with Arc Multi-flow path technology (standard)

Dwell volume selection

Automated with Arc Multi-flow path technology

Solvent conditioning

Integrated vacuum degassing, four chambers

Gradient formation

Low-pressure mixing, quaternary gradient

Gradient profiles

11 gradient curves [including linear, step (2), concave (4), and convex (4)]

Check valves

Passive check valves

Flow accuracy

+/- 1.0% at 0.5, 3.0, and 5.0 mL/min

Flow precision

≤0.075% RSD or +/-0.020 min SD, whichever is greater, based on six replicates [60:40 water:methanol pre-mixed; 1.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm]

Composition ripple

≤0.5 mAU [mobile phase containing 0.1% TFA in water/acetonitrile; 1.5 mL/min; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @214 nm]

Composition accuracy

+/- 0.5% absolute (full scale) from 5 to 95%; 0.5 to 5.0 mL/min [methanol; methanol with 5.0 mg/mL caffeine step gradient; UV @273 nm]

Composition precision

+/- 0.15% RSD or 0.04 min SD, whichever is greater based on six replicate injections [60:40 water:methanol via Auto•Blend Technology; 0.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm]

Compressibility compensation

Automatic and continuous

Priming

Wet priming can run at flow rates up to 10 mL/min

Pump seal wash

Standard

Primary wetted materials

316 L stainless-steel, PPS, fluoropolymer, UHMWPE blend, sapphire, ruby, zirconia, DLC, PEEK, and PEEK blend, titanium alloy

Injection volume range

0.1 to 50.0 μL as standard

Up to 1000.0 μL with optional extension loops

Sample capacity

768 [2x 384-well plate]; or 96 [2 mL vial holders]

Any two of the following:

48-position, 2.00 mL vial holder (total capacity of 96 vials)

96-well plate

384-well plate

48-position, 0.65 mL micro-centrifuge tube plate

24-position, 1.50 mL micro-centrifuge plate

Sample compartment temperature

4.0–40.0 °C, settable in 0.1 °C increments (optional)

Temperature accuracy

+/- 0.5 °C at the sensor

Temperature stability

+/- 1.0 °C at the sensor

Injection needle wash

Integral, active, and programmable

Minimum sample required

3 μL residual, using total recovery 2 mL vials

Accuracy (aspiration)

+/- 0.2 μL

Linearity

>0.999; 0.2–50.0 μL

Precision

<1.0% RSD from 0.5 to 0.9 μL

<0.5% RSD from 1.0 to 4.9 μL

<0.25% RSD from 5.0 to 1000.0 μL

Sample carryover

≤0.002% [Caffeine] under UV conditions

Advanced capabilities

Auto-dilution; auto-addition; load ahead

Primary wetted materials

316 L stainless-steel, gold plated stainless-steel, polyimide, PEEK blend, DLC