2475 Fluorescence (FLR) Detector
The culmination of HPLC sensitivity and selectivity
Waters 2475 Fluorescence (FLR) Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, both naturally fluorescent and derivatized with a fluorescent tag. It sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths and can monitor up to four independent wavelength pairs.
With the 2475 FLR Detector, your lab can achieve high sensitivity, low dispersion methods with unsurpassed accuracy, maximum uptime, and complete confidence in the analysis.
2475 Fluorescence (FLR) Detector
Specifications
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Excitation wavelength range |
200 to 890 nm |
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Emission wavelength range |
210 to 900 nm |
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Bandwidth |
20 nm |
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Wavelength accuracy |
±3 nm (via patented2 Erbium filter) |
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Wavelength repeatability |
±0.25 nm |
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Sensitivity |
S/N, Raman peak of water ≥1000 |
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Measurement range |
0.001 to 100,000.000 emission units (default) |
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Filter time constant, single λ mode |
0.1 to 5.0 s Hamming (default) 0.1 to 99.0 s (optional RC) |
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Filter time constant, dual λ mode |
1.0 to 50.0 s Hamming (default) 1.0 to 99.0 s (optional RC) |
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Data channels |
Up to four 2D channels or one 3D channel |
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Sampling rate |
Up to 20 points/s in single (wavelength) mode 1 point/s in 3D mode |
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Light source |
Xenon lamp, 150 W Warranty: 2000 hour or 1 year (whichever comes first) |
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Flow cell design |
Axially illuminated |
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Flow cell volume |
13 μL |
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Pressure limit |
145 psi (10 bar) |
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Wetted materials |
316 stainless-steel, fused silica, Teflon |
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Power requirements |
100 to 240 VAC |
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Line frequency |
50 to 60 Hz |
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Power consumption |
280 VA (nominal) |
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Inputs |
Four event inputs |
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Outputs |
Four outputs (2 analog, 2 event) |
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Dimensions |
Width: 34.3 cm (13.5 inches) Height: 20.8 cm (8.2 inches) Depth: 61.0 cm (24.0 inches) Weight: 18.1 kg (40.0 pounds) |
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Operating temperature range |
4 to 40 °C (39 to 104 °F) |
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Operating humidity range |
20% to 80%, non-condensing |
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Audible noise |
<58 dBA |
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Part Name |
Part Number |
|
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2475 Multi-λ Fluorescence Detector for Alliance HPLC Systems (analytical flow cell included) |
186247500 |
|
|
2475 Multi-λ Fluorescence Detector for ACQUITY Arc Systems (low-dispersion analytical flow cell included) |
176017009 |
|
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Optional flow cells: |
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Analytical |
13 μL |
205001034 |
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Low-dispersion analytical |
13 μL |
205001554 |
- All performance specifications are measured following a warm-up period of one hour with ambient Δ T ≤ ± 2.0 °C
- U.S. Patent Numbers: 6,423,249 and 6,783,705
Overview
- Increase efficiency with multiple detection modes – 2D, 3D, and on-the-fly spectral scanning
- Benefit from the versatility of a low-dispersion system solution compatible with a wide range of column dimensions
- Achieve better bench-to-bench reproducibility with normalized emission units
Recommended Use: For highly sensitive quantification of very low-level components, such as environmental or food contaminants.
Features Header
Reliable quantification of trace contaminants
When a highly sensitive method is required for quantification of very low-level components, such as environmental or food contaminants, fluorescence detection is the ideal detection solution due to its low limits of repeatable quantification coupled with its high selectivity for analysis in complex matrices.
Innovation driving maximum sensitivity
The 2475 FLR Detector features a unique axially illuminated flow cell design. The excitation energy enters the rectangular flow cell along its long axis and is reflected along this axis by a mirror at the end of the flow cell. This not only minimizes unwanted scattering, but also effectively doubles the flow cell’s path length, as seen in this flow cell diagram. The resulting emission energy exits from the top of the flow cell. This revolutionary design results in less dispersion, less stray light, less volume, and more path length than a traditional cubic flow cell – ultimately delivering greater sensitivity for your fluorescence applications.
Better day-to-day and bench-to-bench reproducibility
Peak responses are available in emission units (EU), as well as energy units. Emission units are normalized to water, a universally available standard, using an automated diagnostic that eliminates peak dependence on gain settings. Factors that normally influence fluorescence measurements, such as lamp or optics degradation, can be compensated by periodically re-normalizing. Additionally, variations in fluorescence responses that occur from one 2475 FLR Detector to another are significantly reduced. Traditionally, variability is observed in the response of individual photo multiplier tubes (PMTs). Using a calibration procedure unique to the 2475 FLR Detector, repeatable PMT performance from one 2475 FLR Detector unit to another is guaranteed.
Intuitive software control
The 2475 FLR Detector is controlled by Waters Empower Software, offering functionality like on-the-fly spectral scanning to optimize emission, and excitation wavelengths as well as timed event programmability to achieve optimal sensitivity, based upon the auto-optimize gain utility. Empower Software offers powerful features that help you turn raw data into reports by providing streamlined data viewing, archival, and retrieval, and fully customizable reporting. Enhanced compliance-ready capabilities maintain security and comply with Federal regulatory standards such as 21 CFR Part 11.
