PeptideWorks Tryptic Protein Digestion Kits
Rapid, automatable, and robust protein digestion workflow
Peptide mapping for biotherapeutic proteins relies on protein digestion which includes the enzymatic treatment of a protein to produce peptide fragments to further characterize specific peptide regions and modifications. The results of protein digests are essential for protein structural characterization, protein identification, and protein modification monitoring including post-translation modifications, product related impurities, and multi-attribute monitoring (MAM) methods. However, sample preparation for peptide mapping is complex, laborious often with long digestion times.
Waters PeptideWorks Tryptic Protein Digestion Kits for manual or automated workflows uniquely provide your lab with high efficiency, reproducible peptide maps in under 2.5 hours—4x faster than average home-brew methods. Achieve a 78% reduction in missed cleavages and a 98% reduction in contaminated autolysis peaks, which can further complicate data processing. When you combine PeptideWorks with Waters MaxPeak Premier Peptide Columns, you maximize sensitivity while minimizing problematic metal adsorption of acidic deamidated peptides in LC-UV and LC-MS chromatography.
Specifications
Overview
- Achieve robust peptide mapping sample preparation in under 2.5 hours
- Achieve improved and automatable desalting with fit-for-purpose Sep-Pak SEC Desalting Cartridges for reproducible and efficient salt removal with high protein recoveries
- Unlock efficient 30-minute tryptic digestion with novel autolysis-resistant high purity RapiZyme Trypsin
- Streamline peptide mapping with one comprehensive kit for manual or automated workflows and eliminate the need to source multiple reagents
Recommended Use: For achieving fast, high-efficiency, reproducible peptide mapping sample preparation.
Easy 3-step protocol for rapid, robust peptide mapping
The entire PeptideWorks protocol can be conducted using three steps— denaturation, reduction/alkylation, desalting and digestion—in under 2.5 hours. Now your lab can achieve quick, clean, and complete in-solution digestions using one comprehensive kit, without the extra steps of immobilized enzymes, which require additional clean up after digestion to remove the insoluble particle and add time to your prep. Combined with Waters MaxPeak Premier Peptide Columns, labs now have the ideal solution to maximize sensitivity while minimizing problematic metal adsorption of acidic deamidated peptides in LC-UV and LC-MS separations.
4x faster digestion times without performance trade-offs
High enzyme to protein ratios (1:5) are faster, but they often lead to high autolysis. RapiZyme Trypsin’s unique autolysis resistance and low missed cleavage unlocks efficient 30-minute digestions, rather than the standard 3-hour digestions (using 1:20). With RapiZyme Trypsin, your lab can reduce the risk of heat-induced artifact peaks like artificial deamidation or oxidation, all without compromising digestion performance, reproducibility, or sequence coverage.
93% reduction in missed cleavages* for high digestion efficiency
With PeptideWorks, your lab can prepare tryptic peptide mapping samples fast. You gain high digestion efficiency without over-digestion of the protein with less than 5% missed cleavages and non-specific cleavages for NISTmAb.
*when compared to leading immobilized trypsin kit
Increase productivity with simple, robust automation
Peptide mapping is even easier with the Andrew+ Pipetting Robot with Extraction+ and OneLab software. Your lab can automate the entire workflow, including desalting and digestion, saving analysts 1-2 hours per day in the laboratory without sacrificing accuracy or reproducibility. Refer to onelab.andrewalliance.com for the PeptideWorks automation protocol.
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