XBridge Columns
Particle stability and performance for methods development
Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis.
XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography.
XBridge Columns
Unwanted interactions between analytes and metal ions?
XBridge Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.
Overview
- Provides high pH stability and chemical resistance for reliability and longevity
- Enables maximum method development flexibility
- Available in a range of BEH column chemistries: C18, C8, Shield RP18, Phenyl, HILIC, and Amide fully scalable 2.5 XP, 3.5, 5 μm analytical, and 10 μm OBD preparative particle sizes and column formats
- Application-specific columns for proteins, peptides, oligonucleotides, and glycan analysis
- Prolongs column lifetimes with optional XBridge VanGuard Cartridges
Recommended Use: For methods development flexibility with improved analyte peak shape and recovery, even under extreme operating conditions.
XBridge Column Featured Technologies
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Rugged hybrid organic/inorganic base particle -
Improved peak shape for bases-low silanol activity
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Wider operating pH range for method development flexibly
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Mechanical strength for maximum column efficiency at UHPLC operating pressures
XBridge BEH Column Characteristics
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General purpose reversed-phase C18 column
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Superior chemical stability pH 1–12
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Elevated operating temperature, up to 90 °C
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Alternative selectivity to traditional alkyl chain C18 columns
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Improved basic analyte peak shape near neutral pH
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Compatible with 100% aqueous mobile phases
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General purpose HILIC column
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Ideal chemistry for the separation of carbohydrates/saccharides
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Excellent chemical stability pH 2–11
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Elevated operating temperature, up to 90 °C
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General purpose reversed-phase column
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Ideal chemistry for the separation of strongly hydrophobic compounds
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Excellent chemical stability pH 1–12
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Elevated operating temperature, up to 90 °C
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Alternative selectivity to C18 chemistries
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Unique selectivity for aromatic compounds
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Excellent chemical stability pH 1–12
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Elevated operating temperature, up to 80 °C
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General purpose HILIC column
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Alternative selectivity to BEH Amide chemistries
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Unique selectivity for strong polar basic compounds
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Excellent chemical stability pH 1–9
XBridge HPLC and UHPLC Application-Specific Column Characteristics
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Suited for analyzing released and 2-aminobenzamide (2-AB) labeled N-linked glycans from various biotherapeutics
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Suited for the analysis of detritylated synthetic oligonucleotides using ion-pair, reversed-phase chromatography
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Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
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Suited for the separation of proteins by size exclusion or by reversed-phase chromatography, maximizing recovery and minimizing protein carryover
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Deliver high resolution size-exclusion chromatography (SEC) separations of mAb, aggregates, monomers, and fragments in less time than many traditional SEC columns
Built with BEH for mechanical strength and chemical stability
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
Highest pH stability
XBridge HPLC and UHPLC Columns offer higher mobile-phase pH stability than any other commercially available chromatographic phase, making it ideal for method development. Unlike approaches that claim high pH resistance, the pH stability of these columns is created by utilizing proprietary surface modifications and is incorporated into the particle synthesis process.
The columns were tested under accelerated pH 10 stability test conditions against some of the most popular chromatographic phases that claim extended high pH stability. The C18 columns showed very little degradation in chromatographic performance, exceeding the performance of the best silica column by more than 1000%.
Preparative separation performance
Waters innovative Optimum Bed Density (OBD) Preparative design ensures direct scalability that is independent of particle size. The combination of the revolutionary OBD format with the BEH base particle delivers the most stable, efficient, and reproducible preparative LC columns available. XBridge OBD Preparative Columns for LC users benefit from high pH and temperature stability for increased mass loading for basic compounds.
Protect your column without compromise
Don’t take chances. Complex sample matrices encountered in pharmaceutical, natural product, environmental, and industrial chemical analysis can prematurely shorten analytical UHPLC and/or HPLC column lifetime and degrade chromatographic performance. Using guard cartridges like VanGuard Protection products improve analytical performance by removing particulate and chemical contamination that may be present in the mobile-phase stream—without compromising chromatographic performance.
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