The BioResolve SEC mAb 7.8 x 150 mm Column contains 2.5 µm BEH particle technology and is application specifically tested with the mAb Size Variant Standard to ensure accurate and highly reproducible quantitation of monoclonal antibody (mAb) monomers from frequently associated high molecular weight aggregates (≥300,000 Da) and lower molecular weight fragments (e.g., ≤100,000 Da). This column is also available with Water mAb Size Variant Standard (PN 176004592) at no extra charge.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
BioResolve |
Product Type |
Columns |
Units per Package |
1 pk |