Preparative HPLC Columns
Increase throughput with preparative productivity and predictability
As a medicinal chemist working on a challenging drug discovery project or purification scientist attempting to maximize yield, your background in chromatographic separations has typically meant working with other chemists to successfully isolate compounds. However, as fewer drugs are approved for market release, your laboratory must refine your drug discovery or purification processes and use more effective methods to increase throughput.
As your lab works to achieve fast, efficient, lab-scale separations and greater throughput, Waters Preparative HPLC Columns can help you attain predictable performance and easily scale from UPLC, UHPLC, or HPLC screening to lab-scale purification. With the proprietary Optimum Bed Density (OBD) design of Waters Preparative HPLC Columns, you can ensure consistency in column-to-column performance and increase productivity through higher recoveries and longer column lifetimes – making lost samples, repeat purification runs, and poor scalability from small to large volume columns a thing of the past.
Preparative HPLC Columns
Specifications
Overview
- Achieve fast, efficient, lab-scale separations for greater throughput
- Directly scale from UPLC, UHPLC, or HPLC screening to lab-scale purification
- Select robust chromatographic particles designed for purification
- Increase productivity through higher recoveries and longer column lifetimes
Recommended Use: For laboratory-scale HPLC purification.
Waters OBD Preparative Columns
XBridge OBD Preparative Columns for LC users benefit from high pH and temperature stability for increased mass loading for basic compounds. Waters innovative OBD preparative design ensures direct scalability that is independent of particle size. The combination of the revolutionary OBD format with the BEH base particle delivers the most stable, efficient, and reproducible preparative LC columns available.
The scalability and versatility of XSelect Charged Surface Hybrid (CSH) Columns makes them powerful method development tools. The hybrid materials used to create the CSH stationary phases are known industry-wide for being the most pressure-tolerant columns on the market. Rugged performance combined with OBD technology produces columns that have exceptional column lifetimes under a wide range of operating conditions. With their scalable design, you can choose from larger to smaller and narrower preparative columns to reduce solvent consumption and fraction volumes.
The OBD design used in SunFire Columns delivers reliable and consistent preparative column performance. SunFire OBD Preparative Columns ensure equivalent chromatographic performance from analytical to preparative dimensions, eliminating the need for subsequent method re-development. SunFire Preparative Particles and columns are manufactured in a cGMP environment, under tight synthesis and column packing controls, ensuring accurate scale-up capability. SunFire OBD Preparative Columns exhibit optimum column efficiencies and lifetime through extended usage of over 1000 DMSO injections.
Atlantis T3 and HILIC Silica Prep OBD Columns offer analytical column performance in preparative chromatography formats to deliver a variety of advantages, including improved bed stability, higher efficiency, longer column lifetime, and increased loadability.
The Atlantis phases are well known for providing superior retention of polar compounds as well as the ability to operate in extreme mobile phase conditions, including 100% aqueous (T3) or highly volatile, >80% organic conditions (HILIC).
XTerra Preparative OBD Chromatography Columns deliver analytical performance in preparative dimensions with maximum column stability thanks to the OBD design and unique chromatographic properties of Hybrid Particle Technology. With these capabilities, new drug candidates can go directly from the lead generation to the lead optimization stage of drug discovery. The ability to operate at high pH provides excellent peak shapes for bases and increased mass loading (up to 60x) as compared to conventional silica-based materials.
Increase preparative column lifetime with OBD technology
The lifetime of your preparative HPLC columns shouldn’t be random. With the extensive development of column hardware and packing process for OBD Preparative Columns, Waters has achieved predictable uniform density throughout the column and maximized mechanical stability. These extended column lifetimes and high mass loading capacity lead to increased productivity for our customers, who have come to rely on the high purity and recovery they can obtain with these columns. Scientists select OBD Preparative Columns for robustness, predictable performance, and ease of scaling from analytical to prep.
Prevent leaks with OBD Preparative Column design
Prevent leaks at high operating pressures with OBD Preparative Columns, which are designed to incorporate a pair of specially designed distributors and chemically-inert seals.
Directly scale UPLC methods to Preparative HPLC Columns
Successful scaling of methods from analytical to preparative dimensions requires the use of chromatographically equivalent columns. All to often, even when the same chemistry phase and particle size are used, methods do not scale either due to loss of resolution and/or lower than expected loading. By matching the analytical and preparative column bed densities, scalability is assured. OBD Preparative Columns are packed to bed densities that closely match the equivalent analytical column, providing excellent stability, reproducibility, and efficiency.
Discover the mass loading capacity for Preparative OBD Columns
Mass capacity of preparative columns can be affected by many factors. In general, mass loading capacity is typically higher for strongly retained material, higher for simple mixtures, lower where higher resolution is required, and they are very strongly dependent on loading conditions. For more detailed instructions on scaling-up, visit our Preparative OBD Column Calculator.
Simplify peptide isolation and purification
Unpredictable separations, short column lifetimes, and lack of scalability can make peptide isolation and purification difficult. Peptide columns provide a stable surface chemistry that can be used for most peptides. Scaling the optimized separation requires identical column chemistry and performance across a range of column dimensions. With OBD Preparative Column design and the stable surface chemistry of Waters peptide columns, in a range of particle sizes, you can improve the efficiency of peptide isolation and purification processes and reduce overall costs. For more information, read our Practical Approaches to Peptide Isolation.
Extend column lifetimes with preparative guards
The use of preparative guards will greatly extend preparative column lifetimes. Guard columns are easy to install ahead of the preparative column and provide superior protection. Waters preparative guard holders and cartridge assemblies are available in 10 mm length in four different inner diameters including 7.8, 10, 19, and 30 mm. These guard holders and cartridge assemblies protect Waters OBD Preparative Columns with the same internal diameter, as shown with these options:
- 7.8 x 10 mm cartridges and 7.8 x 10 mm Cartridge Holder
- 10 x 10 mm cartridges and 10 x 10 mm Cartridge Holder
- 19 x 10 mm cartridges and 19 x 10 mm Cartridge Holder
- 30 x 10 mm cartridges and 30 x 10 mm Cartridge Holder
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