Waters Fraction Manager - Analytical
Uniquely designed to accurately collect fractions from high-efficiency UPLC separations
Efficiency gains realized by ultra-performance liquid chromatography (UPLC) and ultra-high-performance liquid chromatography (UHPLC) separations often get lost with traditional slow-speed, high-volume HPLC-grade fraction collectors.
Minimize fraction loss and carryover to better manage low volume peaks and efficiently collect small amounts of material for further assays with the Waters Fraction Manager (WFM-A), an analytical-scale fraction collector for UPLC systems. Waters has designed the WFM-A to provide high purity and recovery, confidently minimize sample loss, and reduce carryover of your complex mixtures and precious samples.
Waters Fraction Manager - Analytical
Specifications
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Operating UPLC flow range |
0.1 to 2.2 mL/min with standard needle |
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Operating HPLC flow range |
0.1 to 5.0 mL/min with high flow needle |
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pH operating range |
pH 2–12 |
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Internal delay volume |
13.6 µL* |
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Maximum back pressure |
<300 psi |
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Needle move time |
0.7 s Vessel to vessel with needle above vessel 1.1 s Vessel to vessel with needle into bottom of vessel |
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Carryover |
<0.15% with rinse enabled |
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Fraction compartment temperature |
Range 4.0–40.0 °C, settable in 0.1 °C increments; maintains 19.0 °C below ambient with a tolerance range between -2.0 °C–4.0 °C |
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Primary wetted material |
MP35N, PEEK, Teflon, DLC, and Tefzel |
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Integrated leak management |
Leak sensor as standard, and safe leak handling |
*Under specific conditions
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Fraction simulation |
Model and optimize collection parameters using chromatogram data |
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Needle rinse |
Rinse needle before injection or at the end of a peak per user specified duration |
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Needle flush |
Flush needle volume into the last vessel at the end of a time window, after a collection state change, or at end of a peak |
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Plate type (2 plates) |
Capacity |
Total capacity |
|
96-well plate (shallow or deep) |
96 wells |
192 wells |
|
2 mL vial plate |
48 vials |
96 vials |
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4 mL vial plate |
24 vials |
48 vials |
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10 mL vial plate |
15 vials |
30 vials |
|
Eppendorf tube plate (0.65 mL) |
48 vials |
96 vials |
|
Eppendorf tube plate (1.5 mL) |
24 vials |
48 vials |
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Audible noise |
<60 db |
|
|
Operating temperature |
Range 4–40 °C |
|
|
Storage temperature |
-40 °C–60 °C |
|
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Humidity |
20% to 80% non-condensing |
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External control |
Empower® or MassLynx® Software with FractionLynx™ Application Manager |
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External communications |
Ethernet interfacing via RJ45 connection to host PC |
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Event inputs/outputs |
Inject start, auxiliary output |
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Voltage |
90 to 264 VAC |
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Frequency |
47 to 63 Hz |
|
Width |
34 cm (13.5 inches) |
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Height |
27 cm (10.75 inches) |
|
Depth |
71 cm (28 inches) |
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Weight |
20 kg (45 lbs) |
Overview
- Designed specifically to collect the narrow and closely eluting compounds found with UPLC separations.
- Developed on the ACQUITY UPLC platform, the industry standard for innovative and reliable technology, the WFM-A integrates seamlessly into all Waters analytical UPLC/UHPLC and HPLC systems.
- Biocompatible when paired with ACQUITY Premier System with MaxPeak High Performance Surfaces (HPS) Technology collection needle and inert flow path to cool fractions for biopharma applications.
Recommended Use: Ideal for accurately collecting fractions in pharmaceutical, biopharmaceutical, impurity isolation, metabolite, and natural product applications.
Features Header
Purposefully designed for high-efficiency, high-purity collections
The WFM-A overcomes the peak purity challenges of typical fraction collectors and includes features like:
- Low peak dispersion: The low volume, rapid switching valve reduces dispersion and allows the precise collection of narrow UPLC peaks.
- Sample recovery: To further increase recovery of your smallest fractions, the advanced needle design focuses flow and prevents precious sample droplets from forming on the tip.
- Temperature control: The sample compartment is precisely controlled at a range of 4–40 °C to accommodate thermally labile samples.
- Biocompatibility: The WFM-A inert flow path allows effortless integration into your UPLC or HPLC system, accommodating all biomolecule applications.
- System versatility: Specialized needles are designed to accurately dispense fractions for UPLC flow rates of 2 mL/min or higher HPLC flow rates of 5 mL/min.
Novel design for less carryover, maximum recovery, and higher purity
Fraction purity is a major concern when performing analytical fraction collection due to the inherent small volume application work. Classic fraction collector designs trap a portion of the fraction in the dispensing tube when the valve closes. The trapped portion is then collected with the next fraction when it is dispensed typically referred to as “crosstalk.”
The innovative fluidic design of the WFM-A minimizes carryover risk. The syringe can be programmed to dispense a small amount of rinse solvent through the dispense needle after a fraction is collected. This rinse can be directed either to waste, or into the current fraction vessel increasing fraction purity by reducing crosstalk.
Better peak integrity with minimal band broadening
The negligible volume of the WFM-A fraction valve, combined with optimized tubing volumes between the detector and the valve, minimizes band spreading of the fractions. The figure shows that the WFM-A fraction valve peak shape superimposes the straight bypass, whereas the other valves demonstrate much greater dispersion.
The WFM-A effectively collects what you want, when you want it.
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