PATROL UPLC Process Analysis System

Number of solvents:

Up to four, in any combination of two: A1 or A2, and B1 or B2

Solvent conditioning:

Vacuum degassing (six-channels): one channel per solvent, and two channels for the Process Sample Manager (PSM) diluent and sample wash solvents

Gradient formation:

High pressure mixing, binary gradient

Pump seal wash:

Equipped with a programmable active wash system to flush the rear of the high pressure seals and the plungers

Gradient profiles:

11 gradient curves [including linear, step (2), concave (4), and convex (4)]

Primary check valves:

Intelligent Intake Valves (i² Valve)

Flow accuracy:

±1.0% at 0.500 mL/min, as per Waters AQT/SystemsQT protocol

Flow precision:

0.075 %RSD or ±0.01 min SD, (0.500 to 2.000 mL/min), whichever is greater using premixed solvent

Compressibility compensation:

Automatic, no user intervention required

Priming:

Wet priming runs at a flow rate of 4 mL/min

Pump compositional accuracy:

±0.5% absolute (full scale), from 5% to 95% of flow rates from 0.5 to 2.0 mL/min

Pump compositional precision:

0.15 %RSD or ±0.04 min SD, whichever is greater, based on retention time

Primary wetted materials:

316 stainless-steel, UHMWPE, sapphire, ruby, FEP, PTFE, ETFE, diamond-like coating, PEEK and PEEK alloys, titanium alloys

Number of solvents:

One to four, in any combination as standard

Expanded solvent choices with optional six-port solvent select valve

Solvent conditioning:

Integrated vacuum degassing, four chambers

Two additional for the PSM diluent and sample wash solvents

Gradient formation:

Low-pressure mixing, quaternary gradient

Gradient profiles:

11 gradient curves [including linear, step (2), concave (4), and convex (4)]

Primary check valve:

Intelligent Intake Valve (i² Valve)

Flow accuracy:

±1.0% at 0.5 to 2.0 mL/min

Flow precision:

0.075 %RSD or ±0.020 min SD, whichever is greater, based on six 

Composition ripple:

<1.0 mAU (<0.1 mAU with optional 250.0 μL mixer) (baseline noise)

Composition accuracy:

±0.5% absolute (full scale) from 5% to 90% from 0.5 to 2.0 mL/min

Composition precision:

<0.15 %RSD or ±0.04 min SD, whichever is greater, based on six replicate

Compressibility compensation:

Automatic, no user intervention required

Priming:

Wet priming can run at flow rates up to 4 mL/min

Pump seal wash:

Equipped with a wash system to flush the rear of the high pressure seal and the plunger

Flow ramping:

Range: 0.01 to 30.00 min to reach 2.0 mL/min

Default:

0.45 min to reach 2.0 mL/min

Primary wetted materials:

316 L stainless-steel (QSM), or Titanium (bioQSM), and/or PPS, fluoropolymer, fluorelastomer, UHMWPE blend, sapphire, ruby, zirconia, Nitronic 60, DLC, PEEK, and PEEK blend

Unattended operation:

Leak sensors, full 96-hour diagnostic display through console software

Maximum sample capacity:

32 available sites for process and control samples

Internal sample capacity:

32 positions for standard 8 mL vials

External sampling:

1 port standard

Number of sample injections:

Up to 60 injections per sample vial

Sample access:

Total random access

Injection mode:

Full-loop injection only

Injection loop volumes:

1 μL, 2 μL, or 5 μL sample loops only

Dilution range:

Up to 1:100

Injection precision (without dilution):

< or =0.5 %RSD for caffeine peak area, full-loop injection

Injection precision with dilution:

< or =1.0 %RSD for caffeine peak area, full-loop injection

Dilution linearity:

1 to 50 dilution factor > or =0.999 coefficient of deviation

Sample carryover:

1 to 50 dilution factor < or =0.01% of the previous injection for caffeine

Needle wash solvent consumption:

Variable (configured by user)

Sample wash solvent consumption:

Variable (configured by user)

Diluent solvent consumption:

Variable (configured by user)

Minimum injection cycle time:

Variable depending injection mode (configured by user)

Minimum on-line sample volume:

Variable based on injection mode, tubing ID and length required (configured by user)

Maximum at-line sample:

100 μL consumed per analysis (vial)

Sample compartment:

4.0 to 40.0 °C programmable in 0.1 °C increments temperature control

Sample compartment:

±0.5 °C from set point measured at the control sensor temperature stability

Sample compartment:

±1.0 °C from set point measured at the control sensor temperature accuracy

Sample compartment:

±3 °C from set point as measured in air, not in a vial temperature distribution

Sample compartment cooling time:

<60 minutes from 30 to 4 °C