ACQUITY Premier Protein SEC 250Å, 1.7 µm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a “generic” or “platform-type” method for many samples. Note: This column includes the recommended MaxPeak Premier SEC 250Å, 2.5 µm, 4.6 x 30 mm Guard (p/n: 186009969) to help extend column life without sacrifycing desired component resolution. Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
7000 psi (483 Bar) |
Endcapped |
No |
Bonding Technology |
BEH-PEO |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
650,000 |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
250 Å |
QC Tested |
Protein |
Format |
Online Column |
Surface Area |
174 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33, L59 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY |
Product Type |
Columns |
Units per Package |
1 pk |